The frequency of shiga-like toxin (stx1 and stx2) and EHEC-hlyA in food by multiplex PCR
نویسندگان
چکیده
منابع مشابه
Isolation, Characterization and Antibiotic Resistance of Shiga Toxin-Producing Escherichia coli in Hamburger and Evolution of Virulence Genes stx1, stx2, eaeA and hly by Multiplex PCR
Background & Objectives: Shiga toxin-producing Escherichia coli (STEC) O157:H7 have emerged as pathogens that can cause food-borne infections and severe and potentially fatal illnesses in humans. E.coli O157:H7 colonizes the digestive tract of cattle and is transmitted to humans by food and water. The objectives of this study were to characterize the prevalence of E.coli O157:H7 isolates in ham...
متن کاملMultiplex real-time PCR method to identify Shiga toxin genes stx1 and stx2 and Escherichia coli O157:H7/H- serotype.
A multiplex real-time PCR method to simultaneously detect the stx1 and stx2 genes of Shiga toxin-producing Escherichia coli and a unique conserved single-nucleotide polymorphism in the E. coli O157:H7/H- uidA gene has been developed. There is more than 98.6% sensitivity and 100% specificity for all three gene targets based on a panel of 138 isolates. The PCR efficiencies were >/= 1.89, and as f...
متن کاملEvaluation of ehxA, stx1, and stx2 Virulence Gene Prevalence in Cattle Escherichia coli Isolates by Multiplex PCR
Today, it is nearly 25 years past from investigation of Shiga toxigenic Escherichia coli (STEC) which is able to produce Shiga toxins and cause different gastroenteritis. Since incidence of gastroenteritis due to STEC is increasing, it's necessary to develop rapid, specific and accurate procedures like PCR. In this study, we used PCR method to detect and identify STEC in cultures of 55 Escher...
متن کاملCharacterization of Shiga-toxin producing E.coli (STEC) and enteropathogenic E.coli (EPEC) using multiplex Real-Time PCR assays for stx1, stx2, eaeA
BACKGROUND AND OBJECTIVE Diarrheal disease is still a major health problem, especially in developing countries, where it is considered as one of the leading causes of morbidity and mortality especially in children. Studies showed that Diarrheagenic E. coli (DEC) such as STES and EPEC strains are among the most prevalent causative agents in acute diarrhea, particularly in children. Aim of the pr...
متن کاملPrevalence of Virulence Genes of Escherichia Coli O157:H7 Isolated from Patients with Urinary Tract Infections in Shiraz, Iran
Abstract Background and Objective: Escherichia coli O157:H7 is one of the most well-known pathogenic bacteria worldwide that can develop severe diseases such as hemolytic uremic syndrome (HUS). This study aimed to assess the prevalence of virulence genes of E. coli O157:H7 in patients with suspected urinary tract infections (UTIs). Material and Methods: This cross-sectional study was co...
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ژورنال
عنوان ژورنال: Revista Romana de Medicina de Laborator
سال: 2017
ISSN: 2284-5623
DOI: 10.1515/rrlm-2017-0025